The Lipid, Lipoprotein and Atherosclerosis Analysis Core Laboratory at Wake Forest Innovation Quarter has been in operation for over 30 years and has supported analyses in over 300 peer-reviewed publications. Current major offerings in the Lipid, Lipoprotein and Atherosclerosis Analysis Laboratory include:
- Major lipid class (cholesterol, cholesterol ester, phospholipid, and triacylgycerol) quantification after TLC separations in all tissues including blood plasma, red cells, liver, artery, as well as other organs of interest.
- Lipid class fatty acid analysis performed by gas liquid chromatography.
- Plasma and tissue sterol analysis performed by gas liquid chromatography.
- Dietary compositional analysis including lipid class, fatty acid and cholesterol and non-cholesterol sterol compositions.
- Plasma lipoprotein isolation and quantification by gel filtration chromatography with on-line analyses of cholesterol and triglycerides in VLDL, LDL and HDL.
- Isolation of plasma lipoproteins by preparative ultracentrifugation.
- Apolipoprotein analyses of plasma and isolated plasma lipoproteins using Western blotting techniques.
- Identification of diet or drug effects on gene expression in various tissues using RNA extraction, cDNA synthesis and quantitative PCR.
- Quantitative analyses of SOAT1 (i.e. ACAT1) and SOAT2 activities in tissues as well as specific analyses of individual drug effects on either SOAT enzyme in intact cells using transfected cell lines in culture.
- Quantification of atherosclerosis extent using histologic and chemical analyses of various arterial preparations from mice, rabbits, and monkeys.
- Perform studies to evaluate small molecule effects on atherosclerosis using transgenic mouse models.
Learn more about working with the Lipid, Lipoprotein and Atherosclerosis Analysis lab by contacting Wake Forest Innovations at +1.336.713.1111 or firstname.lastname@example.org.