Molecular Partners provides instrumentation and expertise to enable investigators to detect and measure interactions between biological macromolecules using an array of biotechnologies: surface plasmon resonance spectroscopy (SPR), fluorescence spectroscopy, circular dichroism spectroscopy, light scattering and analytical ultracentrifugation.
Measurements of the rate and extent of reversible complex formation between two macromolecules, one immobilized on a biosensor chip and the other delivered by microfluidics, can be performed with a Biacore T100 high-performance research instrument. This system integrates SPR biosensor technology with robotic control of coupling chemistry, analyte delivery, signal acquisition and data processing. The Biacore T100 also provides precise temperature control and powerful data reduction algorithms enabling the acquisition and analysis of real-time kinetic data. Typical applications include measuring the on- and off-rates and equilibrium constants for receptor:ligand, antigen:antibody, protein:nucleic acid, or protein:lipid interactions.
Excitation and emission scans, fluorescence lifetime, and anisotropy measurements can be performed on an ISS K2 Multifrequency Phase Modulation Fluorometer equipped with a Xenon-Hg arc lamp and an argon ion laser. Typical applications include fluorescence anisotropy measurements of the rate and extent of interactions between a fluorophore-tagged ligand or oligonucleotide and a receptor or DNA binding protein.
Circular Dichroism Spectroscopy
CD measurements of macromolecular secondary structure can be performed on a JASCO Model 720A Spectropolarimeter. Typical applications include comparing the solution conformation of a wild-type protein and a series of single-site mutants or measuring denaturant-induced unfolding of a macromolecule.
Classical (static) and dynamic light scattering measurements can be performed with a Malvern Nanosizer. Typical applications include characterizing the oligomeric state of a macromolecule and determining the size distributions of lipid vesicle preparations.
Sedimentation velocity and equilibrium measurements can be performed with a Beckman XLA analytical ultracentrifuge. Typical applications include characterizing the size, shape and oligomeric state of macromolecules.
We encourage you to work with our staff to design, carry out and interpret pilot experiments designed to test the feasibility of an in-depth investigation.